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Whole physique fixation was carried out on animals that have been anesthetized with pentobarbitone as described (Slot et al., 1991a), utilizing a mixture of 2% paraformaldehyde and 0.2% glutaraldehyde in 0.1 M sodium phosphate buffer, pH 7.4.
In some experiments glutaraldehyde was omitted or recent atrial tissue was dissected and fastened by immersion. For cryosectioning (Slot et al., 1991a) small fragments of the fastened tissue were ready from the rim of the left or proper atrium and immersed in 10% gelatin for ∼15 min at 37°C.
The gelatin was then solidified on ice and small gelatin-embedded tissue blocks had been immersed in 2.3 M sucrose at 4°C overnight. The EM sections have been immunolabeled using protein A-gold as the marker for single and double labeling (Slot et al., 1991a).
LM sections had been positioned on poly-l-lysine-coated glass slides and similarly immunolabeled, utilizing CY3-conjugated goat anti-rabbit IgG as a fluorescent marker. Thus, extra research using a extra numerous pattern of classrooms and simultaneous assessments of children’s social integration and detrimental social behaviors, akin to peer battle and aggression, is needed to additional elucidate the moderating function of youngster self-regulation within the relation between ECE process quality and children’s early social skills.